CAS：100068-60-8
Molecular weight：1013.39
Extinction coefficient (cm -1 M -1)：2700001
Excitation (nm)：754
Emission (nm)：778
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He-Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiR iodide [1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg 0.5 mg 1 mg 5 mg 10 mg
1 mM 98.679 µL 493.393 µL 986.787 µL 4.934 mL 9.868 mL
5 mM 19.736 µL 98.679 µL 197.357 µL 986.787 µL 1.974 mL
10 mM 9.868 µL 49.339 µL 98.679 µL 493.393 µL 986.787 µL
染料DiI， DiO， DiD 和 DiR是一类亲脂性荧光染料家族，用于标记细胞膜和疏水性组织。这是一类环境敏感型荧光染料，当它们与膜结合或者与亲脂性生物分子（例如蛋白质，虽然在水中其荧光强度很弱）结合时，其荧光强度显著增强。它们具有很高的淬灭系数，偏光依赖性和很短的激发寿命。一旦应用于细胞中，这种染料会在细胞内质膜中逐步扩散，导致在其浓度条件下，将整个细胞染色。它们不同的荧光颜色：DiI（橙色荧光）、DiO（绿色荧光）、DiD（红色荧光）、DiR（深红色荧光），为活细胞多色彩荧光成像分析和流式细胞术提供了一种便捷的工具。DiO和DiI可以分别与标准的FITC和TRITC滤光器一同使用。其中，DiO可以被633 nm He-Ne激光激发，并且具有比DiI更长的激发和发射光波长，为标记细胞和组织的那些本身就具有本底荧光的染料提供了非常卓越的替代品。DiR在活体成像或者示踪中非常有用，因为它们所发射的红外光可以高效地穿过细胞和组织，并且在红外光范围内，其本底荧光水平很低。
艾美捷细胞膜荧光探针DiR（AAT-22070）基本参数：
中文名称：细胞膜荧光探针DiR,碘化物
英文名字：DiR iodide[1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide]
规格：25mg
光谱学相关参数：Ex=748nm / Em=780nm；其对应分子量大小为1013。39MW。
形式：固体
保存建议：推荐使用常温进行运输。建议在低于-15℃温度下冷冻保存，要求储存环境干燥并避免光照。
普通储备溶液制备：
表1。将碘化二R（1,1-二十八烷基-3,3,3,3,3-四甲基吲哚三碳菁碘）的比质量重建至给定浓度所需的DMSO体积。注意，体积仅用于制备储备溶液。有关适当的实验/生理缓冲液，请参考样本实验方案。
图2：使用DiR（分类号22070）对活HeLa细胞质膜进行染色。图1：使用DiR（22070）对活HeLa细胞质膜进行染色。细胞核用Hoechst 33342（17530）共染色。
细胞膜荧光探针DiR相关文献：
1.亲脂性近红外染料在造血细胞归巢全身光学成像中的应用
作者：Kalchenko V、Shivtiel S、Malina V、Lapid K、Haramati S、Lapidot T、Brill A、Harmelin A。
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