
链霉亲和素与生物素(Biotin)有很高的结合活性,因此被广泛用于检测生物素或生物素偶联的各种蛋白、核酸以及其它分子。
艾美捷Cy7标记链霉亲和素包括链亲和蛋白(作为生物素结合蛋白),Cy7共价连接(作为荧光标记)。该Cy7标记链霉亲和素是使用AAT Bioquest的特别标记技术制备的,可以显示出更亮的信号,因此可以显著提高测定灵敏度。当与生物素化的一级抗体结合使用时,它通常用作间接免疫荧光染色的第二步试剂。它是一种有价值的工具,用于基于生物素-链霉亲和素的生物测定和测试,使用带有Cy7过滤器的流式细胞术。
艾美捷CY7标记链霉亲和素基本参数:
中文名称:Cy7标记链霉亲和素
英文名字:Cy7-streptavidin conjugate
编号:AAT-16914
规格:1mg
描述:Cy7标记的链霉亲和素的光谱学相关参数为Ex=749nm/ Em=776nm;
形式:固体,推荐将其溶解于水中使用或保存。
保存建议:推荐室温运输。建议在低于-20°避光保存,有效期12个月。
图.用(红色,+)或不使用(绿色,-)小鼠抗人HLA-ABC生物素(W6/32生物素)孵育HL-60细胞,然后用Cy7®-链霉亲和素缀合物孵育。在APC-C7通道中使用ACEA NovoCyte流式细胞仪监测荧光信号。
艾美捷CY7标记链霉亲和素文献引用:
Persistent repression of tau in the brain using engineered zinc finger protein transcription factors
Authors: Wegmann, Susanne and DeVos, Sarah L and Zeitler, Bryan and Marlen, Kimberly and Bennett, Rachel E and Perez-Rando, Marta and MacKenzie, Danny and Yu, Qi and Commins, Caitlin and Bannon, Riley N and others,
Journal: Science Advances (2021): eabe1611
Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996
艾美捷CY7标记链霉亲和素参考文献:
A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647