抗双链RNA单克隆抗体J2英文说明书：
Anti-dsRNA monoclonal antibody J2 is the gold standard for the de-tection of dsRNA both in cultured cells and fixed paraffin-embeddedhistological samples.
Applications;
ELISA,IF,FACS,IHC,IP Dot Blot,ChlP,affinity purification,immuno-electron microscopy.
Please note that nucleic acid separation prior to dsRNA-immunoblotting must be carried out by polyacrylamide gelelectrophoresis,because the sensitivity of detection is considerablylower after blotting from agarose gels.
Specificity:
Anti-dsRNA monoclonal antibody J2 recognises double-stranded RNA(dsRNA)provided that the length of the helix is greater than or equalto 40 bp.dsRNA-recognition is independent of the sequence andnucleotide composition of the antigen.All naturally occurring dsRNAsinvestigated up to now(40-50 species)as well as poly(1)-poly(C)andpoly(A)-poly(u)have been recognised by Anti-dsRNA monoclonalantibody J2 although in some assays its affinity to poly(l-poly(C)isabout 10 times lower than that to other dsRNA antigens.
Species Origin:Mouse
Heavy Chain Isotype:IgG2a
Light Chain Isotype:kappa
Quality controL:
Purity/Identity:Reducing and Non-reducing SDS-PAGE
Activity:AN-ELISA(relative activity compared to reference J2)
Related Products:
PCR-grade water,#PCR-258
Shipping:shipped on gel packs
Storage Conditions:store at-20 °C to-80°C upon reconstitution for
long-term storage
Additional Storage Conditions:avoid freeze/thaw cycles,store inaliquot
After adding 10 mM sodium azide the undiluted antibody (1 μg/μl)can be stored at 4°C for a short period of time
Shelf Life:12 months after date of delivery
Form:lyophilised from a 1 mg/ml solution in PBS
Solubility:To prepare a 1 μg/μl PBS antibody solution add 200 μl(RNT-SCl-10010200)or 500 μl(RNT-5C-10010500)sterile
DNAse/RNAse-free distilled water.As a result of the lyophilisationprocedure,the reconstituted antibody may contain small amounts ofdenatured protein in the form of aggregates that may interfere withsome applications such as immunohistochemistry (e.g.by givinghigh backgrounds).We therefore highly recommend to spin down(microcentrifuge)the reconstituted antibody before use and to usethe supernatant only.
抗双链RNA单克隆抗体J2中文说明书：
艾美捷抗双链RNA单克隆抗体J2#RNT-SCI-10010500是检测培养细胞和固定石蜡包埋组织学
物种来源：小鼠
重链亚型：IgG2a
轻链亚型：kappa
质量控制：纯度：还原和非还原SDS-PAGE
活性：与参考J2相比的AN-ELISA（相对活性）
运输：通过凝胶包运送。
存储条件：重组后在-20°C至-80°C下储存以长期保存。
附加存储条件：避免冻融循环，分装保存。
添加10 mM亚硝酸钠后，未稀释的抗体（1 µg/µl）可以在4°C下短期保存。
保质期：自交货日期起12个月。
形态：从PBS中的1 mg/ml溶液中冻干。
溶解性：为制备1 µg/µl的PBS抗体溶液，加入200 µl（RNT-SCI-10010200）或500 µl（RNT-SCI-10010500）无菌DNA/RNAse-free蒸馏水。由于冻干程序，重组后的抗体可能含有少量变性蛋白以聚集体形式存在，这可能会干扰某些应用，如免疫组化（例如，导致高背景）。因此，我们强烈建议在使用前对重组后的抗体进行离心（微离心）处理，并仅使用上清液。
应用：
ELISA、IF、FACS、IHC、IP、点印迹、ChIP、亲和纯化、免疫电子显微镜。请注意，在进行双链RNA免疫印迹前，核酸分离必须通过聚丙烯酰胺凝胶电泳进行，因为从琼脂糖凝胶转印后的检测灵敏度会显著降低。
特异性：
抗双链RNA单克隆抗体J2能够识别双链RNA（dsRNA），前提是螺旋的长度大于或等于40个碱基对。对dsRNA的识别不受抗原的序列和核苷酸组成的影响。到目前为止，所有已研究的自然存在的dsRNA（40-50种）以及聚(I)-聚(C)和聚(A)-聚(U)都被抗双链RNA单克隆抗体J2所识别，尽管在某些实验中，它对聚(I)-聚(C)的亲和力比其他dsRNA抗原低约10倍。
抗双链RNA单克隆抗体J2文献参考：
Schönborn et al. (1991) Monoclonal antibodies to double-stranded RNA asprobes of RNA structure in crude nucleic acid extracts. Nucleic Acids Res.19:2993.
Lukacs (1994) Detection of virus infection in plants and differentiation betweencoexisting viruses by monoclonal antibodies to double-stranded RNA. J. Virol.Methods47: 255.
Lukacs (1997) Detection of sense:antisense duplexes by structure-specificanti-RNA antibodies. In: Antisense Technology. A Practical Approach, C.Lichtenstein and W. Nellen (eds), pp. 281-295. IRL Press, Oxford
