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韶关中英文说明书丨艾美捷R-Phycoerythrin标记链霉亲和素

分类:新闻中心 发布时间:1970-01-01 35次浏览

艾美捷R-Phycoerythrin标记链霉亲和素英文说明书: Informa...

艾美捷R-Phycoerythrin标记链霉亲和素英文说明书:

Information:

Streptavidin, a bacterial protein isolated from Streptomyces avidinii, is similar to egg-white avidin in its ability to bind biotin, and has been used as a replacement for egg-white avidin because of its more favorable chemical properties.

 

Conjugates of streptavidin are recommended for use with Biotin-SP-conjugated antibodies and Biotin-SP-conjugated ChromPure proteins.

 

Usage:

Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Store at 2-8°C – do not freeze. Prepare working dilution on day of use.

Expiration date: six months from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6

Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)

Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

1:50 - 1:200 for most applications

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Conjugate:

R-Phycoerythrin

Amax: 488 Emax: 580nm

 

艾美捷R-Phycoerythrin标记链霉亲和素中文说明书:

链霉亲和素是与亲和素具有相似性质的一种蛋白质,都具有与生物素特异结合的能力,结合常数高达10(15)M.同时,由于链霉亲和素等电点比亲和素低,且不含糖基链,故在检测中的灵敏度和特异性都高于亲和素,建立在此基础上的生物素-链霉亲和素系统的应用比生物素-亲和素系统有更大优势.

 

艾美捷R-Phycoerythrin标记链霉亲和素基本参数:

物理状态:冻干固体

储存和再水合:将冻干固体储存在2-8°C。用规定体积的dH2O(见产品规格表)进行再水化,如果不澄清,则进行离心。储存在2-8°C–不要冻结。使用当天准备工作稀释液。

有效期:自补液之日起六个月。如果试验结果可用于预期用途,则可延长有效期。

缓冲液:0.01M磷酸钠,0.25M NaCl,pH 7.6

稳定剂:15 mg/ml牛血清白蛋白(无IgG、无蛋白酶)

防腐剂:0.05%叠氮化钠

建议工作浓度或稀释范围:

1: 50-1:200适用于大多数应用

稀释因子以范围的形式表示,因为稀释度是许多因素的函数,如抗原密度、渗透性等。实际使用的稀释度必须根据经验确定。

 

艾美捷R-Phycoerythrin标记链霉亲和素可与Biotin标记的各种亲和纯化二抗或者Biotin标记的色谱级蛋白联用进行各种免疫检测.

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与亲和素相比,由于更容易穿透组织并且分子结构更小,链霉亲和素更稳定、更灵敏,并且背景更低.R-Phycoerythrin标记链霉亲和素可以用各种酶联免疫、免疫组化、流式分析、原位杂交以及斑点杂交等实验.

 

来源:https://www.amyjet.com/products/016-110-084.shtml

相关标签: 艾美捷

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