Endothelial cell growth additive (ECGF/ECGS) is an indispensable supplement for endothelial cell culture in vitro, which can optimize the cell growth environment in vitro and promote the normal proliferation and growth of endothelial cells. ECGF/ECGS is a sterile concentrated (100X) solution containing growth factors, hormones and proteins necessary for normal human endothelial cell culture. Endothelial cell growth additives are formulated (quantitative and qualitative) to provide a balanced growth environment, which can well promote the growth of human endothelial cells in vitro.
Emaget Relia Tech endothelial cell growth additive can also be used to culture hybridoma cells without feeder cells (Pintus, 1983). The active factors in these brain extracts have been identified as variants of aFGF (Burgesse, 1985). This ECGF product is supplemented with human rec.VEGF-A of physiological concentration to support the growth and differentiation of vascular endothelial cells in culture.
艾美捷Relia Tech内皮细胞生长添加剂可用于培养没有饲养细胞的杂交瘤细胞（Pintus，1983）。这些脑提取物中的活性因子已被鉴定为aFGF的变体（Burgesse，1985）。该ECGF产品补充了生理浓度的人rec.VEGF-A，以支持培养中血管内皮细胞的生长和分化。
含VEGF 165用于微血管内皮细胞培养：
Cat# 300-090-L：是ECGF pro 2，用于淋巴内皮细胞（HLEC）的培养。ECGF pro 2补充重组人VEGF-C（终浓度：50 ng/ml）。本品也可补充肝素（Cat# 300-090H-L）。
含VEGF-C用于淋巴内皮细胞培养：
ECGF用于人微血管内皮细胞（HMEC）培养的最适浓度范围是100-300 μg/ml，加入肝素（50 μg/ml）后的最适浓度范围是10-20 μg/ml。ECGF pro 1因子和牛ECGF pro 2因子对小鼠细胞，牛细胞和人细胞起作用。
*每瓶中含有足够多的生长因子添加物和VEGF-A或VEGF-C，可以为血液或者淋巴内皮细胞提供最适生长环境。
艾美捷Relia Tech内皮细胞生长添加剂引文赏析：
纯ECGF（Cat# 300-090）
Pharmacological Characterization of the Microsomal Prostaglandin E2 Synthase-1 Inhibitor AF3485 In Vitro and In Vivo. L. Di Francesco et al., Front Pharmacol. 2020; 11: 374.
PDGFRA defines the mesenchymal stem cell Kaposi’s sarcoma progenitors by enabling KSHV oncogenesis in an angiogenic environment. J. Naipauer et al., PLoS Pathog. 2019 Dec; 15(12): e1008221.
Platelet extracellular vesicles induce a pro-inflammatory smooth muscle cell phenotype. T. Vajen et al., J Extracell Vesicles. 2017; 6(1): 1322454.
Endothelial progenitor cells are differentially impaired in ANCA-associated vasculitis compared to healthy controls. B. Wilde et al., Arthritis Res Ther. 2016; 18: 147.
Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels. J. Schniedermann et al., BMC Cell Biol. 2010; 11: 50.
含肝素ECGF（Cat 300-090H）
Kaposi’s sarcoma herpesvirus activates the hypoxia response to usurp HIF2α-dependent translation initiation for replication and oncogenesis. O. Méndez-Solís et al., Cell Rep. 2021 Dec 28; 37(13): 110144.
艾美捷Relia Tech内皮细胞生长添加剂相关研究：
RET-300-090 ECGF（粗提取物，细胞培养级）6ug
RET-300-090-5 ECGF（粗提取物，细胞培养级）5x6mg
RET-300-092 ECGF（粗提取物，细胞培养级）6ug
RET-300-092-5 ECGF（粗提取物，细胞培养级）5x6mg
RET-300-090H ECGF（粗提取物，细胞培养级）+肝素6mg
RET-300-090H-5 ECGF（粗提取物，细胞培养级）+肝素5x6mg
RET-300-092H ECGF（粗提取物，细胞培养级）+肝素6mg
RET-300-092H-5 ECGF（粗提取物，细胞培养级）+肝素5x6mg
RET-300-090-B ECGFpro1（完全用于血液ECs）6mg
RET-300-090H-B ECGFpro1+肝素（完全用于血液ECs）6mg
RET-300-090-L ECGFpro2（完整用于淋巴ECs）6mg
RET-300-090H-L ECGFpro2+肝素（完全用于淋巴ECs）6mg
