荧光底物，用于测定20S蛋白酶体、钙蛋白酶以及其他类胰凝乳蛋白酶的胰凝乳蛋白酶样肽酶活性（激发波长：380 nm；发射波长：460 nm）。

艾美捷Suc-LLVY-AMC，蛋白酶体底物：

货号：PS-0500-5000  

规格：5 mg  

分子量：763.9  

分子式：C₄₀H₅₃N₅O₁₀  

状态：冻干粉  

纯度：薄层色谱法检测 >98%  

溶解性：在DMSO中至少可溶至5 mM，浓度范围为10-100 µM  

储存：干粉于4°C保存；DMSO溶液于-20°C保存。避免反复冻融。  

应用：  

测定20S蛋白酶体的胰凝乳蛋白酶样肽酶活性。  

测定钙蛋白酶及其他类胰凝乳蛋白酶的肽酶活性。  

筛选蛋白酶体和肽酶抑制剂。

文献参考：

1. Tsubuki S et al. (1993) Purification and characterization of a Z-Leu-Leu-Leu-MCA degrading protease expected to regulate neurite formation: a novel catalytic activity in proteasome. Biochem. Biophys. Res.Commun. 196, 1195

2. Stein RL. et al. (1996) Kinetic characterization of the chymotryptic activity of the 20S proteasome.Biochem. 35:3899-3908

3. Dang LC. et al. (1998) Kinetic and mechanistic studies on the hydrolysis of ubiquitin C-terminal 7-amido4-methylcoumarin by deubiquitinating enzymes Biochemistry 37, 1868

4. Wang KK et al. (1996) An alpha-mercaptoacrylic acid derivative is a selective nonpeptide cell-permeable calpain inhibitor and is neuroprotective. Proc. Natl. Acad. Sci. USA 93, 6687

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