异硫氰酸荧光素FITC是生化试剂,也是医学诊断药物,主要用于荧光抗体技术中的荧光染料,能和各种抗体蛋白结合,结合后的抗体不丧失与一定抗原结合的特异性,并在碱性溶液中具有强烈的绿色荧光。用于医学,农学和畜牧等方面,可对由地细菌病毒和寄生虫等所致疾病进行快速诊断。
异硫氰酸荧光素标记用试剂，多肽、蛋白、抗体标记用试剂，氨基标记用荧光试剂，含异硫氰基的荧光试剂。
艾美捷异硫氰酸荧光素相关参数：
中文名称：异硫氰酸荧光素
英文名字：FITC [FITC Isomer I; fluorescein-5-isothiocyanate]
货号：B-CHM100-100mg
规格：100mg
产品形式：固体
保存建议：异硫氰酸荧光素储存条件：-20℃干燥避光保存，有效期一年。
纯度：高于或等于95%（通过高效液相色谱法测定）
光谱特性：激发/发射波长 = 490 / 525 纳米
外观：黄色粉末
异硫氰酸荧光素标记抗体流程：
(1) Dialyze the protein to be cross-linked (concentration: 1 mg/mL) against the cross-linking reaction buffer threetimesat 4°C, adjusting the pH to 9.0. Preparation of the cross-linking buffer: Dissolve 7.56 g NaHCO3, 1.06 g Na2CO3, and7.36g NaCl in water to a final volume of 1 L.
(2) Dissolve FITC in DMSO at a concentration of 1 mg/mL. Prepare fresh FITC solution for each cross-linking reactionandavoid exposure to light.
(3) Add FITC slowly to the antibody solution at a ratio of P:F (protein:FITC) = 1 mg:150 μg. Gently mix the solutionwhileadding FITC to ensure uniform mixing with the antibody. Allow the reaction to proceed at 4°C in the dark for 8hours.
(4) Add 5 mol/L NH4Cl to a final concentration of 50 mmol/L to stop the reaction at 4°C for 2 hours.
(5) Dialyze the conjugates against PBS solution at least four times until the dialysate becomes clear.
(6) Characterization of the cross-linked material:Protein concentration (mg/mL) = [A280 - 0.31 × A495] / 1.4F/P ratio: 3.1 × A495 / [A280 - 0.31 × A495]. This value should be between 2.5 ~ 6.5.
(7) Store the FITC protein conjugates in a PBS at pH 7.4, supplemented with 0.1% NaN3 and 1%BSA, storeat 4°Cinthe dark
