Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis. Oxidation of arachidonic acid by prostaglandin synthases (COX-1 and COX-2) produces prostaglandin H2 (PGH2), which is further metabolized by PGE synthases into its major product, PGE2. PGE2 mediates autocrine and paracrine signaling by binding to G-protein coupled receptors (EP1, EP2, EP3, EP4) on the cell surface, functioning to modulate phospholipase C and adenylate cyclase activity. PGE2 has been of great interest as a therapeutic target, either by modulation of its synthesis by COX inhibitors (NSAIDS) or by modulation of its receptors by downregulation or binding antagonists. PGE2 production in a variety of tissues has been shown to modulate numerous physiological processes including natriuresis in the kidney, smooth muscle elasticity in the vasculature, and the inflammatory response to damaged tissues by monocytes and macrophages.
Sensitivity: 13.4 pg/ml (range 39.1 - 2,500 pg/ml)
Assay Time: <3 hours
Applications: ELISA, Colorimetric detection
Application Notes: For the quantitative determination of PGE2 in culture supernatants, serum, saliva, urine, and whole blood from any species. Cited sample types include cerebral spinal fluid, dialysate, gingival crevicular fluid, peritoneal exudate fluid and peritoneal exudate cell supernatant, plasma, and tissue.
Wavelength: 405 nm
Species reactivity: Species independent
Use/Stability: Store all components at +4°C, except standard and conjugate at -20°C.
Shipping: Shipped on Blue Ice
Contents: GxM IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
PGE2酶联免疫试剂盒：用于炎症和类二十烷研究的高灵敏度PGE2 ELISA试剂盒。
PGE2酶联免疫试剂盒化学性质：
替代名称：前列腺素E2
灵敏度：13.4 pg/ml（范围39.1-2500 pg/ml）
化验时间：<3小时
应用：ELISA，比色检测
应用说明：用于定量测定任何物种的培养上清液、血清、唾液、尿液和全血中的PGE2。引用的样本类型包括脑脊髓液、透析液、龈沟液、腹膜渗出液和腹膜渗出细胞上清液、血浆和组织。
波长：405 nm
物种反应性：物种无关
使用/稳定性：在+4°C下储存所有成分，-20°C下标准和共轭物除外。
运输：蓝冰运输
内容：GxM IgG微量滴定板、缀合物、抗体、测定缓冲液、洗涤缓冲液浓缩液、标准品、pNpp底物、停止溶液
PGE2酶联免疫试剂盒文献参考：
Leptin reduces in vitro cementoblast mineralization and survival as well as induces PGE2 release by ERK1/2 commitment: G. Ruiz-Heiland, et al.; Clin. Oral. Investig. 25, 1933 (2021), Abstract; Full Text
Potential Surrogate Quantitative Immunomodulatory Potency Assay for Monitoring Human Umbilical Cord-derived Mesenchymal Stem Cells Production: S. Teng, et al.; Cell Biol. Int. 45, 1072 (2021), Abstract;
Synergistic effect of Korean red ginseng extract and GABA mixture on the IgE production in mice via Th1/Th2 cell balance: J.S. Lim, et al.; Food Sci. Biotechnol. 30, 1571 (2021), Abstract;
The inhibitory effect of AMP-activated protein kinase (AMPK) on chemokine and prostaglandin production in human endometrial stromal cells: Y. Kawano, et al.; Reprod. Biol. Endocrinol. 19, 188 (2021),
