The 12(S)-HETE EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 5 hours. Absorbance is read at 405 nm. Fast, accurate, and reliable results are obtained with ready-to-use liquid color-coded reagents that reduce error.
Easy-to-use color-coded reagents and pre-coated plates reduce errors and save time in the lab
High throughput format, obtain results from up to 39 samples in duplicate in just 5 hours
Highly sensitive assay, measure as little as 146 pg/ml
Reliable and reproducible results day-after-day and lot-after-lot
Sensitivity: 146 pg/ml (range 195 - 50,000 pg/ml)
Assay Time: 5 hours
Applications: ELISA, Colorimetric detection
Application Notes: For the quantitative determination of 12(S)-HETE in culture supernatants and plasma from any species. Cited sample types include serum, renal interstitial fluid and tissue.
Wavelength: 405 nm
Species reactivity: Species independent
Use/Stability: Store all components at +4°C, except standard and conjugate at -20°C.
Shipping: Shipped on Blue Ice
Contents: GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
12（S）-HETE对多形核白细胞和血管平滑肌细胞具有趋化性和化学动力学。它也作为血管紧张素II诱导的醛固酮产生的第二信使。
12(S)-HETE-ELISA试剂盒是一种比色竞争性酶免疫测定试剂盒，5小时内即可获得结果。在405 nm处读取吸光度。使用易于使用的液体色码试剂可获得快速、准确和可靠的结果，从而减少误差。
艾美捷12(S)-HETE-ELISA试剂盒——灵敏的ELISA试剂盒能够检测来自任何物种的培养上清液和血浆中的12（S）-HETE。
12(S)-HETE-ELISA试剂盒化学性质：
替代名称：12（S）-氢过氧四烯酸二十碳四烯酸
灵敏度：146 pg/ml（范围195-50000 pg/ml）
化验时间：5小时
应用：ELISA，比色检测
应用说明：用于定量测定任何物种培养上清液和血浆中的12（S）-HETE。引用的样本类型包括血清、肾间质液和组织。
波长：405 nm
物种反应性：物种无关
使用/稳定性：在+4°C下储存所有成分，-20°C下标准和共轭物除外。
运输：蓝冰运输
内容：GxR IgG微量滴定板、缀合物、抗体、测定缓冲液、洗涤缓冲液浓缩液、标准品、pNpp底物、停止溶液
12(S)-HETE-ELISA试剂盒艾美捷特点：
1.易于使用的色码试剂和预涂板减少了错误并节省了实验室时间
2.高通量格式，仅需5小时即可获得多达39份样本的结果，一式两份
3.高灵敏度测定，测量值低至146 pg/ml
4.日复一日、一批又一批可靠且可重复的结果
12(S)-HETE-ELISA试剂盒文献参考：
12-Lipoxygenase Inhibition on Microalbuminuria in Type-1 and Type-2 Diabetes Is Associated with Changes of Glomerular Angiotensin II Type 1 Receptor Related to Insulin Resistance: H.Z. Xu, et al.; Int. J. Mol. Sci. 17, E684 (2016), Application(s): Measurement of HETE levels in rats, Abstract; Full Text
Cancer cell-derived 12(S)-HETE signals via 12-HETE receptor, RHO, ROCK and MLC2 to induce lymph endothelial barrier breaching: C.H. Nguyen, et al.; Br. J. Cancer 115, 364 (2016), Application(s): 12(S)-HETE assay, rabbit, Abstract; Full Text
12/15-Lipoxygenase metabolites of arachidonic acid activate PPARγ: a possible neuroprotective effect in ischemic brain: L. Sun, et al.; J. Lipid. Res. 56, 502 (2015), Application(s): ELISA, Abstract; Full Text
Lobatin b inhibits NPM/ALK and NF-κB attenuating anaplastic-large-cell-lymphomagenesis and lymphendothelial tumour intravasation: I. Kiss, et al.; Cancer Lett. 356, 994 (2015), Application(s): ELISA using MCF7 cell culture supernatants,
